- Title
- PEA-CLARITY: 3D molecular imaging of whole plant organs
- Creator
- Palmer, William M.; Martin, Antony P.; Flynn, Jamie R.; Reed, Stephanie L.; White, Rosemary G.; Furbank, Robert T.; Grof, Christopher P. L.
- Relation
- Scientific Reports Vol. 5
- Publisher Link
- http://dx.doi.org/10.1038/srep13492
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2015
- Description
- Here we report the adaptation of the CLARITY technique to plant tissues with addition of enzymatic degradation to improve optical clearing and facilitate antibody probe penetration. Plant-Enzyme-Assisted (PEA)-CLARITY, has allowed deep optical visualisation of stains, expressed fluorescent proteins and IgG-antibodies in Tobacco and Arabidopsis leaves. Enzyme treatment enabled penetration of antibodies into whole tissues without the need for any sectioning of the material, thus facilitating protein localisation of intact tissue in 3D whilst retaining cellular structure.
- Subject
- plant tissue; antibodies; molecular interrogation; three dimensional
- Identifier
- http://hdl.handle.net/1959.13/1332429
- Identifier
- uon:26867
- Identifier
- ISSN:2045-2322
- Rights
- This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
- Language
- eng
- Full Text
- Reviewed
- Hits: 2902
- Visitors: 3189
- Downloads: 317
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 845 KB | Adobe Acrobat PDF | View Details Download |